Nicotiana tabacum l . nc89 ' amp; jyh 對2個(gè)不同基因型煙草品種
Doubling efficiencies of the application dimethylsurfoxide in colchicines to treat haploids in tobacco nicotiana tabacum 對煙草單倍體植株的染色體加倍效應(yīng)
Ssmapkk showed highest homology to the npk2 gene from nicotiana tabacum . genomic southern blot analysis suggested there was only one copy ssmapkk gene in suaeda salsa genome Ssmapkk與已報(bào)道的煙草npk2同源性最高, southern雜交表明該基因在鹽地堿蓬基因組中只有一個(gè)拷貝。
Acbf is a transcription factor that combined with the ac - rich region of the promoter region . it has been cloned at arabidopsis thaliana , nicotiana tabacum and so on Acbf編碼了結(jié)合在苯丙氨酸解氨酶啟動(dòng)子ac富集區(qū)上的一個(gè)轉(zhuǎn)錄因子, acbf基因已經(jīng)在擬南芥、煙草等植物中得到了克隆。
Finally , the full length cdna of real differential fragments were cloned by performing both 5 ' - and 3 ' - rapid amplification of cdna ends ( race ) and then the functional screening was performed by transferring the full length cdna into arabidopsis thaliana or nicotiana tabacum plant 108號克隆尚未做測序分析。通過race技術(shù)對152和233兩個(gè)差異片段進(jìn)行全長cdna克隆,獲得了152的全長cdna序列和233的3 ’ 。 cdna序列。
Leaves of tobacco ( nicotiana tabacum ) were wounded , infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0 . 5mg / l 6 - ba + 0 . 7 % agor ph 5 . 7 ) 4以煙草(品種sr )無菌苗葉片為外植體,采用農(nóng)桿菌浸染的葉盤轉(zhuǎn)化法,用構(gòu)建好的植物表達(dá)載體對煙草進(jìn)行遺傳轉(zhuǎn)化。外植體置于無抗生素的誘芽培養(yǎng)基( ms 0
In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ) , nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence 在用煙草作為生物反應(yīng)器時(shí),分別將該單鏈抗體靶向細(xì)胞質(zhì)和內(nèi)質(zhì)網(wǎng)。經(jīng)westernblot分析,靶向細(xì)胞質(zhì)中表達(dá)時(shí),可溶單鏈抗體最高占總的可溶蛋白的0 . 06 。
Northern blot results show that nos . 66 - 1 , 84 , 89 - 1 , 97 , 108 , 152 , 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no . 23 has weak signal only in sp6 - tester , nos . 94 , 165 , 172 , 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos . 4 , 17 , 18 , 28 , 6 9 , 101 , 156 - 1 , 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no . 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76 , and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180 . 1 , supported by cdna : 7119 , supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no . 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685 , gi | 2605931 | gb | af029898 . 1 | af029898 ) for aspartate aminotransferase ; no . 89 - 1 cdna has significant alignment with arabidopsis tha 與同源性最高的擬南芥類似晚期胚胎發(fā)生高豐度蛋白比較,二者都具有l(wèi)ea 2結(jié)構(gòu)域、保守分泌蛋白cog5608結(jié)構(gòu)域和低復(fù)雜度區(qū),都具有pkc磷酸化位點(diǎn)、酪蛋白激酶磷酸化位點(diǎn)、 n十四酞化位點(diǎn)和酚胺化位點(diǎn),所不同的是: ( )在結(jié)構(gòu)功能域上, 152全長cdna編碼的蛋白質(zhì)序列中多了1個(gè)lea 2結(jié)構(gòu)域、 l個(gè)保守分泌蛋白cog5608結(jié)構(gòu)域和1個(gè)低復(fù)雜度區(qū); ( 2 )在功能位點(diǎn)上, 152全長cdna編碼的蛋白質(zhì)具有酪氨酸硫酸化位點(diǎn)、多了l個(gè)酪氨酸激酶磷酸化位點(diǎn)和1個(gè)可能的天冬氨酸富集區(qū),但沒有n糖基化位點(diǎn); ( 3 )擬南芥類似晚期胚胎發(fā)生高豐度蛋白的lea 2結(jié)構(gòu)域具有顯著性( e
Three binary expression vectors were constructed , which harbored the cl - i - 2kl , r - 2kl , and d - l - 2kl - r - 2kl respectively , driven by the camv 35s promoter . then , agrofeac / erium - mediated transformation of iobacco ( nicotiana tabacum , w38 ) was carried out . transformed tobacco lines were obtained through tissue culture and confirmed by pcr and southern blotting analysis 通過分子克隆技術(shù),將c1 - i - 2k1 、 r - 2k1和二者的連接( c1 - i - 2k1 ? r - 2k1 )分別構(gòu)建到雙元表達(dá)載體上,并使它們分別置于35s啟動(dòng)子的驅(qū)動(dòng)之下,然后通過農(nóng)桿菌介導(dǎo)的技術(shù)分別對煙草( nicotianatabacum , w38 )進(jìn)行了轉(zhuǎn)化,利用組織培養(yǎng)技術(shù)再生植株。
百科解釋
Nicotiana tabacum, or cultivated tobacco, is a perennial herbaceous plant. It is found only in cultivation, where it is the most commonly grown of all plants in the Nicotiana genus, and its leaves are commercially grown in many countries to be processed into tobacco.